Fig. 5.

Dox increased broad H3K27ac deposition at the promoters of cardiotoxicity-related genes. A Overview of H3K27ac CUT&Tag experiment. B Peak number of H3K27ac in DMSO and Dox group. C Heatmap and quantification showing the global H3K27ac signal in DMSO and Dox group, two-tailed Wilcoxon test. D The distribution of signal-increased H3K27ac peak upon Dox treatment. E Heatmap and quantification showing the increased ChIP signal at narrow H3K27ac peak loci in DMSO and Dox group, two-tailed Wilcoxon test. F, G Heatmap and quantification showing the increased ChIP signal at broad H3K27ac peak loci in DMSO and Dox group, two-tailed Wilcoxon test. H Gene ontology analysis showing the biological processes for all the genes with increased H3K27ac signal upon Dox treatment. I, J Gene ontology analysis showing the biological processes for the genes with increased broad (I) and narrow H3K27ac (J) signal upon Dox treatment, and the apoptotic process was highlighted. K–M The representative regions showing the H3K27ac signal around Bcl2l11, Bax and Fas genes between the DMSO and Dox group with two replicates