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MHV-S esterase is able to de-O-acetylate free and glycosidically bound Neu4,5Ac2. Samples in the upper and lower reversed-phase C18 HPLC chromatograms of fluorescent derivatives of free sialic acids were treated with heat-inactivated and active MHV-S, respectively. (A) Free Neu4,5Ac2 (peak 3). Peak 2 represents Neu5Ac. (B) Sialic acids released from guinea pig serum glycoconjugates after treatment with MHV-S. Peak 1, Neu5Gc; peak 2, Neu5Ac; and peak 3, Neu4,5Ac2.
