|
| Antibodies |
|
| Polyclonal Guinea Pig Anti-Insulin |
Agilent - Dako |
Cat# IR00261–2; RRID: AB_2800361 |
| Monoclonal Mouse Anti-Glucagon |
Sigma-Aldrich |
Cat# G2654; RRID: AB_259852 |
| AlexaFluor® 488 Goat Anti-Guinea Pig |
Thermo Fisher Scientific - Invitrogen |
Cat# A-11073; RRID: AB_2534117 |
| AlexaFluor® 633 Goat Anti-Mouse |
Thermo Fisher Scientific - Invitrogen |
Cat# A-21050; RRID: AB_2535718 |
|
| Biological samples |
|
| Human Pancreas Tissue |
nPOD;https://npod.org/
|
RRID: SCR_014641 |
| nPOD-6540 |
|
RRID: SAMN25652251 |
| nPOD-6543 |
|
RRID: SAMN25652254 |
| nPOD-6544 |
|
RRID: SAMN25652255 |
| nPOD-6546 |
|
RRID: SAMN25652257 |
| nPOD-6547 |
|
RRID: SAMN25652258 |
| nPOD-6548 |
|
RRID: SAMN25652259 |
| nPOD-6550 |
|
RRID: SAMN25652261 |
| nPOD-6551 |
|
RRID: SAMN25652262 |
| nPOD-6552 |
|
RRID: SAMN30386842 |
| nPOD-6553 |
|
RRID: SAMN30386843 |
| nPOD-6556 |
|
RRID: SAMN30386845 |
| nPOD-6558 |
|
RRID: SAMN30386847 |
| nPOD-6559 |
|
RRID: SAMN30386848 |
| nPOD-6560 |
|
RRID: SAMN30386849 |
| nPOD-6562 |
|
RRID: SAMN30386850 |
| nPOD-6563 |
|
RRID: SAMN30386851 |
| nPOD-6569 |
|
RRID: SAMN38117299 |
| nPOD-6571 |
|
RRID: SAMN33284289 |
| nPOD-6573 |
|
RRID: SAMN33284291 |
| nPOD-6575 |
|
RRID: SAMN33284293 |
| nPOD-6578 |
|
RRID: SAMN33284295 |
| nPOD-6579 |
|
RRID: SAMN33284296 |
| nPOD-6582 |
|
RRID: SAMN38117302 |
| nPOD-6583 |
|
RRID: SAMN38117303 |
| nPOD-6584 |
|
RRID: SAMN38117304 |
| nPOD-6586 |
|
RRID: SAMN38117306 |
| nPOD-CV14 |
|
RRID: SAMN38117313 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Aprotinin |
MilliporeSigma |
Cat# A6106 |
| Low Melting Point Agarose |
MilliporeSigma |
Cat# A0701 |
| Low Glucose DMEM |
MilliporeSigma |
Cat# D6046 |
| Fetal Bovine Serum |
Cytiva |
Cat# SH30071.03IH |
| Antibiotic-Antimycotic Solution |
Corning |
Cat# 30–004-CI |
| DMEM (Powder) |
Corning |
Cat# MT9013PB |
| Sodium Pyruvate |
MilliporeSigma |
Cat# P2256 |
| HEPES |
MilliporeSigma |
Cat# H4034 |
| L-Glutamine |
Thermo Fisher |
Cat# 25030081 |
| Bovine Serum Albumin |
MilliporeSigma |
Cat# A6003 |
| Carbamylcholine chloride |
MilliporeSigma |
Cat# C4382 |
| Triton X-100 |
MilliporeSigma |
Cat# 648462 |
| 4% Paraformaldehyde |
Thermo Fisher Scientific |
Cat# J19943.K2 |
| PBS with Azide |
Santa Cruz Biotechnology |
Cat# SC-296028 |
| DAPI |
Sigma-Aldrich |
Cat# D9542 |
|
| Critical commercial assays |
|
|
|
| Insulin ELISA Kit |
Mercodia |
Cat# 10-1113-10 |
| Ultrasensitive Insulin ELISA |
Mercodia |
Cat# 10-1132-01 |
| Glucagon ELISA Kit |
Crystal Chem |
Cat# 81520 |
| Human Pancreatic Amylase ELISA Kit |
Abcam |
Cat# ab137969 |
| Human Pancreatic Lipase ELISA Kit |
Novus Biologicals |
Cat# NB016815 |
| Trypsin RIA |
ALPCO |
Cat# 72-OCFE07-TRYPS |
|
| Software and algorithms |
|
|
|
| Prism 9.4.0 |
GraphPad |
RRID: SCR_002798 |
| ImageJ |
https://imagej.nih.gov/ij/
|
RRID:SCR_003070 |
| MorphoLibJ |
https://imagej.net/plugins/morpholibj
|
N/A |
| FIJI |
https://imagej.net/software/fiji/
|
RRID:SCR_002285 |
| Las X Life Science Microscope Software Platform |
https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/
|
RRID:SCR_013673 |
|
| Other |
|
|
|
| Perifusion System |
Biorep Technologies |
Cat#PERI4–115 |
| Semiautomatic Vibratome VT1200S |
Leica |
Cat#14048142065 |
| Perifusion Chamber |
Warner Instruments |
Cat#64–0223 |
| Perifusion Platform |
Warner Instruments |
Cat#64–0281 [P-5] |
| Laser Scanning Confocal Microscope |
Leica |
Model: TCS SP8 SMD |
