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Recovery of spiked Ang-(1-12). Spiked angiotensin-(1-12) (Ang-[1-12]) is recovered fully when using the 6 mol/L guanidine hydrochloride collection approach (A), but disappears rapidly when using nonstabilized plasma incubated at 37 °C, and cannot be detected in nonstabilized plasma during incubation at 37 °C when no exogenous Ang-(1-12) has been added (B). GHC indicates guanidine hydrochloride; and LLOQ, lower limit of quantification.
