Figure 4.

Metabolism of Ang-(1-12) added to human plasma. A, Remaining percentage (mean±SD) of angiotensin (Ang)-(1-12) (1000 ng/mL) added to 1:10 diluted human plasma incubated at 37 °C for 60 minutes in the presence or absence of various inhibitors. **P<0.01, ****P<0.001 vs no inhibitors. B, Angiotensin metabolites (pg/mL) generated in 1:10 diluted human plasma incubated at 37 °C, following the addition of 1000 ng/mL Ang-(1-12) in the presence of aliskiren or aliskiren + lisinopril. ACE indicates angiotensin-converting enzyme; AP, aminopeptidase; DAP, dipeptidyl aminopeptidase; LLOQ, lower limit of quantification; NEP, neprilysin; and ZPP, Z-Pro-Prolinal.