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. 2023 Dec 31;11(27):2306716. doi: 10.1002/advs.202306716

Table 1.

QCM‐D monitoring of the direct biofunctionalization of GFP nanobody‐spyCatcher using spyTag Cysteine (N‐terminal) or spyTag‐Cysteine (C‐terminal). Three replicates were used.

SpyDirect N′ SpyDirect C′
spyTag Nanobody‐spyCatcher spyTag Nanobody‐spyCatcher
Gained mass [ng cm−2] 148 ± 37 526 ± 127 253 ± 11 737 ± 213

Molecule density

[cm−2]

42 × 1012 9 × 1012 86 × 1012 13 × 1012