Table 2.

The amount of peptide, receptor, blocker, as well as target and interferent protein binding on HDT SAM‐ and SpyDirect C′‐functionalized gold surfaces. The averages and the standard deviations are derived from three independent QCM‐D experiments.

	HDT SAM‐based strategy		SpyDirect C′ strategy
Components introduced in each step	Gained mass [ng cm−2]	Molecules [cm−2]	Gained mass [ng cm−2]	Molecules [cm−2]
1) spyTag‐peptide	94 ± 32	3.2 × 1013	241 ± 9	8.2 × 1013
2) VHH72‐spyCatcher	313 ± 26	6.5 × 1012	376 ± 18	7.8 × 1012
3) BSA (monomer)	90 ± 42	8.2 × 1011	43 ± 26	3.9 × 1011
4) non‐target GFP	<4	–	<1	–
5) SARS‐CoV‐2 spike	290	2.3×1012	659	5.2 × 1012