FIG. 3.

Persistence of Ad/RSVhAAT DNA genomes in various murine strains. (A) Southern blot of 2.5 μg of mouse liver DNA prepared from B6 (C57Bl/6), C3H, Balb/c, and Balb.B mice at 9 or 40 days after transduction with 5 × 10⁹ PFU of Ad/RSVhAAT. Bars on the right indicate positions of the unique adenovirus DNA fragment which contains hAAT and the MMT gene fragment. The dot on the left corresponds to signals for the control fragment (10 and 1 hAAT cDNA copies per cell). The image is a dye sublimation print of the PhosphorImager file used for quantification which was labeled and aligned with Canvas 5.0 (Deneba Software, Miami, Fla.). (B) Graphic representation of average intensities of the signals in each of the groups from the blot following detection on a PhosphorImager. The error bars represent standard deviations.