FIG. 8.
Identification of apoptotic hepatocyte nuclei following transduction with Ad/RSVhAAT. Liver sections from Balb/c mice 5, 9, and 40 days after transduction with 5 × 109 PFU of Ad/RSVhAAT (A to C, respectively), a Balb/c positive control mouse 2 h after administration of 10 mg of Jo-1 anti-Fas antibody (D), a Balb.B mouse 9 days after transduction with 5 × 109 PFU of Ad/RSVhAAT (E), and a naive Balb/c negative control mouse (F) were TUNEL stained with FITC to visualize apoptotic nuclei. Shown are images of representative sections, visualized by fluorescence microscopy with a 20× objective, that were assembled and labeled with Canvas 5.0 (Deneba Software). The arrows indicate FITC-positive apoptotic nuclei.