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. 2023 Oct 19;42(7):1107–1117. doi: 10.1038/s41587-023-01945-y

Fig. 6. Discovery of neoepitope-specific TCRs demonstrates immunogenic potential of discovered neoepitope–HLA pairs.

Fig. 6

Human CD8+ T cells were transfected with either FLT3-p.D835Y-specific or PIK3CA-p.E545K-specific TCR RNA. a–c, CD137 expression was assessed after T cells transfected with FLT3-p.D835Y-specific TCRs were co-cultured overnight with YIMSDSNYV peptide-pulsed A*02:01+-T2 cells (a). CD137 expression (b) and specific lysis (c) were determined after co-culture with A*02:01+-K562 cells transfected with no RNA, transgene containing mutant FLT3-p.D835Y or transgene containing FLT3-D835 wild-type sequence. The box represents the interquartile range; the line represents the median value; and the whiskers represent the minimum and maximum values (excluding outliers) (n = 4). d–f, CD137 expression was assessed after T cells transfected with PIK3CA-p.E454K-specific TCRs were co-cultured overnight with STRDPLSEITK peptide-pulsed A*11:01+-K562 cells (d). CD137 expression (e) and specific lysis (f) were determined after co-culture with A*11:01+-K562 cells transfected with no RNA, transgene containing mutant PIK3CA-E545K or transgene containing PIK3CA-E545 wild-type sequence. The box represents the interquartile range; the line represents the median value; and the whiskers represent the minimum and maximum values (excluding outliers) (n = 3). mut, mutant; wt, wild-type.