Fig. 1. Oocytes contain a large number of very long-lived proteins.
a, Fully labelled 13C6-Lys females were mated and fed 13C6-Lys chow until they gave birth (pulse). Upon birth, the pups were transferred to unlabelled foster mothers (chase) and continued to receive 12C6-Lys chow after weaning. In total, 4,948 oocytes were collected from 92 eight-week-old pubertal female progeny and processed for bottom-up MS. b, Selected pathways enriched with long-lived proteins in oocytes. All 13C6-Lys-labelled proteins detected after 8 weeks were subjected to over-representation analysis. Shown are the percentage of genes of the gene set detected as enriched with their respective adjusted P values for selected pathways. The P values are based on a hypergeometric test and have been adjusted using Benjamini–Hochberg multiple hypothesis testing. For the complete list of enriched pathways with their corresponding exact P values, see Supplementary Table 2. c, Selected biological processes and protein complexes with long-lived proteins. Shown are selected genes with corresponding protein %H values (inferred fraction of 13C6-Lys, mean value over biological replicates). For a complete list of proteins and their corresponding %H values, see Supplementary Table 1.