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. 2024 Jun 20;26(7):1124–1138. doi: 10.1038/s41556-024-01442-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — (a) Schematic representation of sample preparation for MS DIA or DDA analysis. (b) Box plot showing the volume (µm³) of oocytes in fixed primordial follicles and at germinal vesicle stage from live and fixed samples. Boxplots indicate median, 1st quartile, 3rd quartile, as well as minimum and maximum after outlier removal. Number of oocytes indicated in parenthesis. (c) Venn diagram of ¹²C₆-Lys containing proteins and ¹³C₆-Lys containing proteins detected in mouse oocytes. (d) Rank plot showing inferred remaining fraction of ¹³C₆-Lys labelled proteins (%H) in oocytes for each quantified protein (individual proteins denoted as black circles). Selected examples are highlighted in red. (e) Bar plot showing number of proteins with %H of 0–1, 1–50, 50–90, or 90–100%. Source numerical data are available in source data.

Source data