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. 2024 Jul 3;15:1404119. doi: 10.3389/fphar.2024.1404119

FIGURE 6.

FIGURE 6

LRE protected IMG cells from oxidative stress by enhancing the expressions of antioxidant enzyme related genes. (A) Cytotoxicity assay of IMG cells treated by H2O2 at various concentrations, including 50, 100, 200, 300, 400, and 800 µM. (B) Cytotoxicity assay of IMG cells treated by LRE at various concentrations, including 100, 200, 400, and 800 μg/mL. (C) The effects of 2 h of pretreatment with LRE at 10, 50, 100, and 200 μg/mL on the cytotoxicity induced by 300 µM H2O2 in the IMG cells. (D) Pretreatment and simultaneous treatment but not post-treatment with 200 μg/mL LRE significantly reduced 300 µM H2O2-induced cytotoxicity in the IMG cells. (E) 2 h of 200 μg/mL LRE treatment on the IMG cells significantly upregulated the gene expressions of GPX-4 and Prdx-5. (F) Pretreatment with 200 μg/mL LRE significantly upregulated HO-1 and SOD-2 gene expressions in the IMG cells with or without the 300 µM H2O2 stimulation. *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs. DMEM control. ##, p < 0.01; ###, p < 0.001 vs. H2O2 stimulation.