Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 16;15:5987. doi: 10.1038/s41467-024-50290-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a The phosphorylation of OsCTR2 decreases in response to ethylene. Etiolated seedlings of the wild-type (WT) were exposed to 10 μL/L ethylene for varying durations. b OsCTR2 phosphorylation is recovered after the removal of ethylene (ET). Etiolated seedlings of the WT were treated with 10 μL/L ethylene for 120 min, followed by sampling at different time points after ethylene removal. c The phosphorylation state of OsCTR2 in mhz3-1 is compared with that in WT, Osein2-1 and Oseil1. Etiolated seedlings were treated with air, 10 μL/L ethylene, and 10 μL/L 1-Methylcyclopropene (1-MCP) for 1 hour. d Functional complementation of mhz3-1 was achieved by transforming the mutant with MHZ3 genomic DNA. Etiolated seedlings grown for 3 days with or without 10 μL/L ethylene are shown, scale bars = 10 mm. Coleoptile and root lengths are means ± SD, n = 30 biologically independent plants (**P < 0.01; two-tailed Student’s t-test; compared with WT). e OsCTR2 phosphorylation is rescued in mhz3-1/gMHZ3 plants. Two-day-old etiolated seedlings were treated with or without 10 μL/L ethylene for 1 hour. f Schematic diagrams of the mutation sites of the allelic mutants of MHZ3. g The OsCTR2 phosphorylation state in mhz3 allelic mutants. Total proteins were extracted from 2-day-old etiolated seedlings of WT, Osers2^d (OsERS2 gain-of-function mutation), and mhz3 allelic mutants. h Overexpression of MHZ3 (MHZ3-OE) in various transgenic plants. MHZ3’s protein content was evaluated utilizing the anti-MHZ3 antibody. i MHZ3 enhances the phosphorylation of OsCTR2. Two-day-old etiolated seedlings were treated with 10 μL/L ethylene for 1 hour, while air-treated seedlings’ protein extracts were treated with λ-Protein Phosphatase (λ-PPase) for 0.5 hours. j λ-PPase treatment resulted in the removal of OsCTR2 phosphorylation. OsCTR2-P denotes the phosphorylated OsCTR2 protein, while OsCTR2 represents the non-phosphorylated form. Additionally, OsCTR2-P^super indicates further phosphorylation modifications in MHZ3-OE plants. OsCTR2 phosphorylation was detected using anti-OsCTR2 antibody, with Binding Immunoglobulin Protein (BiP) as the ER membrane marker for internal reference. Three independent experiments were repeated with similar results. Uncropped blots and source data are in the Source Data file.