Fig. 2. Ethylene receptors differentially regulate OsCTR2 phosphorylation.
a the phosphorylation state of OsCTR2 in various ethylene receptor mutants. Two-day-old etiolated seedlings were treated with air, 10 μL/L ethylene, or 10 μL/L 1-MCP for 1 hour, respectively. Protein extracts were analyzed using the anti-OsCTR2 antibody to assess the phosphorylation state of OsCTR2. Notably, Osers2d is characterized by a dominant mutation leading to the gain of function of OsERS2, whereas the other ethylene receptor mutants exhibit loss-of-function mutations. b Membrane association of OsCTR2 in WT, Osetr2, Osetr2 ers2, and Osetr2 etr3. Equal amounts of total (T), soluble (S), and microsomal membranes (M) proteins were subjected to immunoblotting for OsCTR2, BiP (an ER membrane marker), and UDP-glucose pyrophosphorylase (UGPase, a cytoplasmic marker). Three independent experiments were repeated with similar results. Uncropped blots in Source Data file.