FIG. 4.

Serum and fecal antibody responses in rabbits immunized twice parenterally with buffer (mock), SA11, G3 and G1 2/4/6/7-VLPs sequentially, or G1 2/4/6/7-VLPs and challenged orally with ALA (G3). Prechallenge (■) and postchallenge (□) serum and fecal samples were assayed by ELISA for total serum antirotavirus antibody (A), rotavirus-specific IgG fecal antibody (B), or rotavirus-specific IgA fecal antibody (C). The error bars represent 1 standard error of the mean. The GMTs of the rabbits immunized sequentially with the G3 and G1 or the G1 2/4/6/7-VLPs are compared to the GMTs for mock- and SA11-immunized animals as previously reported (7). Significant increases in GMT postchallenge are indicated by * (P < 0.05).