TABLE 1.
Induction of broadly heterotypic serum neutralizing antibody in mice immunized parenterally with VLPs
| Inoculum | P serotype | G serotype | Neutralizing GMTa (range) of sera against indicated test virus (Pb G type)
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wa (P1A,G1) | S2 (P1B,G2) | SA11 (P5B,G3) | VA70 (P1A,G4) | OSU (P9,G5) | NCDV (P6,G6) | 69M (P4,G8) | WI61 (P1A,G9) | |||
| 2/6/7-VLPsc | NAd | 1 | 12,800 (6,400–25,600) | 800 (800) | 606 (<100–3,200) | 50 (<100) | 57 (<100–100) | 800 (400–1,600) | 459 (<100–800) | 1,600 (800–3,200) |
| 2/4/6/7-VLPsc | 5B | 1 | 102,400 (102,400) | 174 (100–400) | 1,838 (1,600–3,200) | 50 (<100) | 50 (<100) | 400 (400) | 6,400 (6,400) | 696 (400–800) |
| 2/6/7-VLPsc | NA | 3 | 919 (800–1,600) | 459 (400–800) | 102,400 (102,400) | 50 (<100) | 87 (<100–200) | 1,056 (800–1,600) | 50 (<100) | 528 (400–800) |
| 2/4/6/7-VLPsc | 5B | 3 | 919 (800–1,600) | 919 (400–1,600) | 58,813 (51,200–102,400) | 50 (<100) | 87 (<100–200) | 919 (400–1,600) | 50 (<100) | 919 (800–1,600) |
| 2/4/6/7-VLPse | 5B | 1/3 | 3,200 (800–6,400) | 459 (200–800) | 7,352 (1,600–12,800) | 87 (<100–200) | 76 (<100–400) | 1,838 (1,600–3,200) | 50 (<100) | 1,213 (800–1,600) |
a
For titers of <100, 50 was used to calculate the GMT. A GMT of 50 is considered negative.
b
P serotype is indicated (18).
c
Serum neutralizing antibody titers measured by FFN in sera collected 114 dpi from five mice/group immunized three times with VLPs with Freund’s adjuvant.
d
NA, not applicable.
e
Serum neutralizing antibody titers measured by FFN in sera collected 42 dpi in five mice/group immunized twice with chimeric G1/G3 2/4/6/7-VLPs with Freund’s adjuvant.