TABLE 2.
Induction of mucosal heterotypic neutralizing antibody by G1 VLPs
| VLP | P serotype | G serotype | Fecal antibody titera (range)
|
||
|---|---|---|---|---|---|
| ELISA GMTb (range)
|
NAb at 75 dpic | ||||
| 61 dpi | 75 dpi | ||||
| 2/6/7 | NAd | 1 | 30 (<10–80) | 30 (<10–160) | 200 |
| 2/4/6/7 | 5B | 1 | 24e (10–160) | 40 (20–80) | 100 |
| 2/6/7 | NA | 3 | 8 (<10–20) | 34e (10–160) | 100 |
| 2/4/6/7 | 5B | 3 | 17 (<10–40) | 28e (<10–80) | 100 |
a
Five mice/group were immunized parenterally 0, 14, and 61 dpi with the indicated VLPs with Freund’s adjuvant. Fecal samples were collected 61 and 75 dpi and assayed for total antibody (IgA, IgM, and IgG) by ELISA. Nonimmunized mice housed in the same room remained serum and fecal antibody negative throughout the experiment.
b
For titers of <10, 5 was used to calculate the GMT. A GMT of 5 was considered negative.
c
The 75-dpi fecal samples for each group were pooled and tested by PRN for neutralizing antibody (NAb) against G3 SA11.
d
NA, not applicable.
e
The antibody titer of one mouse was not tested due to insufficient fecal sample.