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. 2024 Jul 16;7:867. doi: 10.1038/s42003-024-06559-x

Fig. 7. Gly463 mutations also confer resistance of EcThrRS to BN.

Fig. 7

a Chemical structure of borrelidin (BN). b Superimposition of the structures of EcThrRS_WT–OB (PDB code: 8H98, marine cartoons) with EcThrRS_WT–BN (PDB code: 4P3P, gray cartoons). The RMSD is 0.396 Å over 345 Cα atoms. c Diagram of the ΔTm values of EcThrRS_WT/G463S/G463A in the presence or absence of BN. Error bars represent the standard deviations (n  =  4, mean value  ±  SD). All the data points are shown as small circles. d Inhibitory curves of BN on the ATP hydrolysis activity of EcThrRS_WT, G463S or G463A. Error bars represent the standard deviations (n  =  4, mean value  ±  SD). All the data points for EcThrRS_WT/G463S/G463A are shown as pale blue dots, pale pink square dots, and pale red triangle dots.