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. 2024 Mar 22;14(7):3068–3085. doi: 10.1016/j.apsb.2024.03.025

Figure 5.

Figure 5

Defective E. coli phagocytosis in SLC12A4-defecient cells. (A) Phagocytosis of E. coli by BMDMs transfected with control or SLC12A4-overexpression (SLC12A4-OE) plasmids (n = 4/group). (B) Percentage of E. coli killing by BMDMs transfected with control or SLC12A4-overpression (SLC12A4-OE) plasmids (n = 4/group). (C) Phagocytosis of E. coli by BMDMs transfected with Slc12a4 siRNA or control siRNA for 72 h in the presence or absence of rhamnose (100 μmol/L) (n = 4/group). (D) Percentage of E. coli killing by BMDMs transfected with Slc12a4 siRNA or control siRNA for 72 h in the presence or absence of rhamnose (100 μmol/L) (n = 4/group). (E) Representative confocal images showing phagocytosis of GFP-E. coli in BMDMs transfected with SLC12A4 overexpression or control plasmids for 48 h in the presence or absence of rhamnose (100 μmol/L). Scale bar = 10 μm. (F) Representative confocal images showing phagocytosis of GFP-E. coli in BMDMs transfected with Slc12a4 siRNA or control siRNA for 72 h in the presence or absence of rhamnose. Scale bar = 10 μm. (G) Phagocytosis of E. coli in THP-1 cells transfected with control or SLC12A4-overpression (SLC12A4-OE) plasmids (n = 4/group). (H) Percentage of E. coli killing in THP-1 cells transfected with control or SLC12A4-overpression (SLC12A4-OE) plasmids (n = 4/group). (I) Phagocytosis of E. coli in THP-1 cells transfected with Slc12a4 or control siRNA for 48 h in the presence or absence of rhamnose (100 μmol/L) (n = 4/group). (J) Percentage of E. coli killing in THP-1 cells transfected with Slc12a4 or control siRNA for 48 h in the presence or absence of rhamnose (100 μmol/L) (n = 4/group). (K) Representative confocal images showing phagocytosis of GFP-E. coli in THP-1 cells transfected with SLC12A4 overexpression or control plasmids for 48 h in the presence or absence of rhamnose (100 μmol/L). Scale bar = 10 μm. (L) Representative confocal images showing phagocytosis of GFP-E. coli in THP-1 cells transfected with Slc12a4 or control siRNA for 48 h in the presence or absence of rhamnose. Scale bar = 10 μm. Data are expressed as mean ± SEM. One-way analysis of variance (ANOVA) analysis was used for three or more groups. Two groups were determined by a two-tailed unpaired Student's t-test. ∗P < 0.05 was considered statistically significant. ns, denotes not significant. n: indicates number of samples.