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Overlap between Hyps predicted by three methods and measured hydroxylation status of prolines. Hyps in all secretory Physcomitrella proteins with a signal peptide (SignalP 5) were predicted using the ragp tool, the glycomodule and the extended prolyl-hydroxylation code. Depicted is the overlap between predicted Hyps with measured Hyps (a) and prolines not measured to be hydroxylated (b).
