Fig. 1.

Study design and mutation distribution. (A) Study flowchart of enrollment process and treatment scheme. (B) Percentage of somatic variants/pathologic somatic variants shared among different numbers of patients in 83 osteosarcoma patients. (C) Percentage of somatic variants/pathologic somatic variants shared among different numbers of patients in 59 osteosarcoma patients included. (D) Mutation oncoplot based on WES results in 59 osteosarcoma patients included. (E) Heatmap of the variant allele frequency in WES and ctDNA sequencing for cases 9 and 33.