FIG. 6.

Comparison of polysomal and nonpolysomal RNA localization. RPA of nuclear, polysomal, and nonpolysomal RNAs. RNAs were harvested 2 days posttransfection and subjected to DNase treatment, and 5- to 15-μg aliquots were subjected to RPA with uniformly labeled antisense HIV and gapdh RNA probes, PAGE, and PhosphorImager analysis. Labels indicate the protected RNAs. (A) RPA of RNA from cells transfected with pSVgagpol-rre without and with pCMVRev and pSVgagpol-rre-MPMV. (B) RPA of RNA from cells transfected with pYW99 and pYW100.