Skip to main content
. 2024 Jul 3;121(28):e2320655121. doi: 10.1073/pnas.2320655121

Fig. 6.

Fig. 6.

KCTD10 and USP18 regulate ferroptosis through SLC7A11. (A–C) MDA-MB-231 cells were transfected with indicated siRNAs (si-NC or si-KCTD10s), followed by cystine deprivation (A), treatment with RSL3 (B), and Erastin (C) alone or in combination with Ferr-1 for 24 h as indicated, then cell viability was measured (mean ± SD, n = 3). (D) MDA-MB-231 cells transfected with indicated siRNAs (si-NC or si-KCTD10s) for 24 h, then treated without and with Erastin for 48 h, followed by cell viability measurement (mean ± SD, n = 3). (E–G) MDA-MB-231 cells were transfected with indicated siRNAs (si-NC or si-USP18s), followed by cystine deprivation (E), treatment with RSL3 (F) and Erastin (G) alone or together with Ferr-1 for 24 h, as indicated, followed by cell viability measurement (mean ± SD, n = 3). (H) MDA-MB-231 cells transfected with indicated siRNAs (si-NC or si-USP18s) and plasmid (HA-SLC7A11) for 24 h, then treated without and with Erastin for 48 h, followed by cell viability measurement (mean ± SD, n = 3).