Fig. 2.

Mitochondrial homeostasis was out of balance in the chorionic villi of patients with EA. (A–K) The expression levels of nucleus-encoded proteins (mitofilin, SDHA, COXIV), as well as mitochondrial fusion (MFN1, and OPA1) and fission (DRP1, Fis1, MFF) proteins in the chorionic villi were assessed using WB analysis in both the EA group and the control group. GAPDH and ACTIN were used as a loading control. (L–Q) IHC staining was performed to detect the expression of MFN1, DRP1, OPA1, Fis1, and MFF in human chorionic villi from both the EA group and the control group. Scale bar: 100 μm. Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.