Fig. 5.

Overexpression of HTR1B inhibited cell proliferation and migration, disrupted mitochondrial homeostasis, and overactivated mitophagy in HTR-8/SVneo cells. (A) q-PCR assays for mRNA levels of htr1b in HTR-8/SVneo cells. GAPDH served as a loading control. (B) Wound healing experiments were conducted in both groups of HTR-8/SVneo cells. Scale bar: 200 μm. (C) Cell proliferation was assessed at various time points following HTR1B transfection. (D–G) Cellular mitochondrial function was assessed following HTR1B overexpression. The protein levels of nucleus-encoded proteins (SDHA) and mitochondrial dynamic-related proteins (OPA1and Fis1). (H–L) The mRNA levels of sdha, cox iv, opa1, fis1, and drp1. (M–Q) The protein levels of mitophagy-associated proteins, such as PINK1, Parkin, Beclin1, and the ratio of LC3 II/I. The Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.