FIG. 1.
HPV-16 L1 MAbs V5 and E70 inhibit in vitro infection of HPV-16. HPV-16Rochester-1k/ur3/ur3 was preincubated with the indicated dilutions of E70, U4, or V5 ascites fluid prior to infection of cultured keratinocytes. As a control, virus was also preincubated with the HPV-11-specific MAb F1, which was previously demonstrated not to neutralize HPV-16. Seven days postinfection, cells were harvested, and the presence of the HPV-16 E1^E4 spliced message, diagnostic of HPV-16 infection, was detected by RT-PCR (top). PCR amplification of cellular β-actin was performed with all cDNA samples as an internal control (bottom).