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Total RNA from transfected 3T3 cells was reverse transcribed and PCR amplified by using Mx2 primers (top) and β-actin primers (bottom). Lanes: 1, 3T3 cells; 2 and 3, control 3T3-pCI-neo; 4 and 5, 3T3 cells expressing Mx2 from SPR (S1 and S2); 6 and 7, 3T3 cells expressing Mx2 from NJL (N2 and N4). An aliquot of each PCR product was electrophoresed on a 1.2% agarose gel and visualized with ethidium bromide.
