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. 2024 Jun 24;15(7):e01158-24. doi: 10.1128/mbio.01158-24

Fig 1.

Six-panel graph shows Luc DNA copies under different treatments. Includes melittin and IP6 effects, varying IP6 concentrations, IP5 concentrations, IS6 concentrations, HCB concentrations, and inositol concentrations. Error bars represent variability.

IP6 promotes ERT in MLV. (A) The bar graph shows ERT product formation in the presence and absence of different reagents as indicated in the x-axis of the graph. The y-axis represents absolute copies of luciferase DNA reverse transcribed from the MLV-derived luciferase vector RNA packaged in MLV. In one sample, 30 mM AZTTP was included in the reaction mixture. Below the bar graph is an agarose gel showing amplification of a longer product (luciferase coding sequence, 1,500 bp) after ERT. NTC—no template control for the PCR reaction. (B) IP6 titration in ERT assay. The graphs represent the mean ± SD of three replicates in the qPCR measurement of a single experiment selected from at least two independent experiments with similar results. (C–F) ERT product accumulation by titrating different potential co-factors in place of IP6: (C) IP5, (D) IS6, (E) HCB (mellitic acid), and (F) inositol.