Fig 6.
REC-DBD interface substitutions relieve inhibition and accelerate phosphorylation. (A–B) Autophosphorylation kinetics of BT4124 domains and corresponding interface variants. Phos-tag gels (A) were quantified to track the fraction of phosphorylated proteins (B) at indicated times after addition of 20 mM PAM. (C–D) Comparison of phosphorylation efficiency for RR and REC-DBD interface variants of BT4124 (C) and BT4663 (D). Error bars indicate the standard deviation calculated as described.
