Extended Data Fig. 2. Propionate and vitamin B₁₂ supplementation selectively increase vancomycin resistance of PDIM(+) Mtb improving assay robustness and reducing time to result.

a, Vancomycin MICs for the PDIM reference strain set in standard 7H9/OADC/glycerol/tyloxapol + PALM media and additionally supplemented with 0.1 mM propionate. Growth was measured after 7, 10, and 14 days as indicated. Mean ± SD for n = 4 biological replicates from two independent experiments. b, Vancomycin MICs in standard media and additionally supplemented with 0.1 or 1.0 mM propionate or 7.4 μM vitamin B₁₂. Growth was measured after 10 days. Mean ± SD for n = 4 biological replicates from two independent experiments. c, VAN10 assays in standard and supplemented media. Growth was measured after 10 days. Mean ± SD for n = 3 independent experiments, each performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; two-way ANOVA with Šidák’s multiple comparison test. The day seven data in (a) are also shown in Fig. 1c and are shown here alongside additional time points. The data in (b) includes one of the same experiments shown in (a), together with data from an independent experiment. The VAN10-P (+ 0.1 mM propionate) data in (c) are also shown in Fig. 1e and are shown here alongside additional conditions.