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. 2024 Jun 13;15(7):e01321-24. doi: 10.1128/mbio.01321-24

Fig 6.

Interaction between miR-200a-3p and ZBTB20 mRNA with 3 prime UTR of ZBTB20 mRNA and the location where the miR-200a-3p seed region binds is featured with graphs of RLU across WT UTR and MUT UTR for scramble and mimic, and scramble and inhibitor.

miR-200a-3p regulates ZBTB20 post-transcriptionally by targeting 3′ UTR region of ZBTB20 mRNA. (A) Illustration of the miR-200a-3p sequence, its seed region at 3′ UTR of ZBTB20, and the pmiRGlo construct carrying the 3′ UTR at its multiple cloning site. (B) Bar graph showing the dual luciferase activity of pmiRglo construct carrying the wild-type/mutated ZBTB20 UTR in the presence of scramble/miR-200a-3p mimic. (C) Bar graph showing the dual luciferase activity of pmiRglo construct carrying the wild-type/mutated ZBTB20 UTR in the presence of scramble/miR-200a-3p inhibitor. Data represented as mean fold change ± SEM with respect to control from a minimum of three independent experiments. P-values were determined (*, P < 0.05; **, P < 0.01; ***, P < 0.00) using Student’s t-test.