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. 2024 Jan 4;14(6):100932. doi: 10.1016/j.jpha.2023.12.022

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© 2024 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Subcellular localization of fluorescein isothiocyanate (FITC)-mussel oligosaccharide (MOS-1) in HepG2 cells. (A) HepG2 cells were treated with FITC-MOS-1 (green) and Lyso-Tracker Red (LTR; red). Areas of co-localization are visible in yellow, some of co-localization area were marked by white rectangles named 1−4. (B) Zoom-in images are an area surrounded by the white rectangles numbered 1−4 in Fig. 3A.. (C) Fluorescence intensity profiles of linear regions marked in Fig. 3B (green: FITC-MOS-1 and red: LTR). (D) Three-dimensional (3D) fluorescence distribution maps of FITC-MOS-1 and LTR in HepG2 cells. (E) Co-localization values for FITC-MOS-1 and LTR (n = 8). (F) Area and diameter analyses of FITC-MOS-1 and LTR in a population of cells (n = 30). n.s.: no statistically significant.