Figure 5.
Machine learning analysis of relationship between different AAV serotypes
(A) Cladogram showing phylogenetic classifications of AAV serotypes, based on Gao et al.53 and Mietzsch et al.53,54 (B) Results of Ward’s hierarchical clustering of AAV NAb titers showing the relationship between the six analyzed AAV serotypes. AAV1 and AAV6 formed a cluster, indicating that they are closely related. AAV8 and AAVRh74var also formed a cluster, which was found to be related to AAV5. AAV9 was least similar to the other AAVs. (C) Principal-component analysis (PCA) of normalized NAb titers in all participants for the six AAV serotypes. AAVs that are nearer one another in the two-dimensional projection are more closely related. As observed with the clustering analysis (B), AAV1 and AAV6 were closest to one another, followed by AAVRh74var, AAV8, and AAV5, while AAV9 was most distant from all the other AAVs. AAV, adeno-associated virus; Nab, neutralizing antibody; PC, principal component.