FIG. 2.

Translation of RNA transcripts containing genotype 1a HCV-H or genotype 1b HCV-N 5′NTR sequences. (A) SDS-PAGE of products of translation from rabbit reticulocyte lysates programmed with 2 (lanes 1 and 2), 1 (lanes 3 and 4), 0.5 (lanes 5 and 6), 0.25 (lanes 6 and 8), or 0 (lane 9) μg of RNA derived from pH-CΔE1 (containing the 5′NTR of HCV-H; lanes 1, 3, 5, and 7) or pN-CΔE1 (containing the 5′NTR of HCV-N; lanes 2, 4, 6, and 8) per 25-μl reaction. (B) Quantitative analysis (relative PhosphorImager band volume) of HCV capsid (left) and ΔE1 (right) proteins produced in reticulocyte lysates programmed with increasing concentrations of RNA transcribed from pH-CDE1 (■) or pN-CΔE1 (□).