FIG. 5.

Quantitative, single-cell, indirect immunofluorescence cytometry for HCV capsid and CAT proteins, following infection of Huh-T7 cells with vTF7-3 and transfection with plasmid DNAs containing nearly genome-length HCV sequences with the 5′NTRs of HCV-N or HCV-H (N, pCAT/N; H, pCAT/H) (see text) or pCAT/N derivatives containing the indicated nucleotide substitutions present in the HCV-H 5′NTR (Fig. 1). Each point represents the ratio of capsid (TRITC label) to CAT (FITC label) content for a single cell. Horizontal bars depict means ± standard deviations (values shown below each data set). P values indicate significant differences from values obtained in cells transfected with pCAT-N-CΔE1.