TABLE 1.
79.9 selection conditions
| Round | tRNA concn (μM) | 79.9 pool concn (μM) | Rex (pmol) | Stem IID concn (μM) | Prefiltration | Dilution |
|---|---|---|---|---|---|---|
| 1 | 1.3 | 1.3 | 1.8 | 3× | ||
| 2 | 6.6 | |||||
| 3 | 0.9 | |||||
| 4 | 2.2 | |||||
| 5 | 0.9 | 3× | ||||
| 6 | 3.4 | |||||
| 7 | 4.2 | Yesa | ||||
| 8 | 13 | 8.6 | Yesb |
a
Diluted to a final volume of 1 ml in 1× binding buffer. This reaction was allowed to equilibrate an additional 10 min and then filtered.
b
Diluted to 800 μl in 1× binding buffer, and then an additional 200 μl of XRE-containing solution was added for a final concentration of 6.9 μM. This reaction was allowed to equilibrate an additional 10 min and then filtered.