Figure 2.

Hydrazide resin approach for purification of linear peptide aldehydes from a complex mixture. (a) General scheme for the capture of linear peptide aldehydes from a mixture using hydrazide resin and the release of pure linear peptide aldehydes in solution. (b) Structure of model peptide 1a with stacked chromatograms (DAD) of the crude model peptide 1a, the filtrate collected after hydrazide resin scavenging, and the filtrate after peptide hydrolysis from hydrazide resin to generate pure peptide aldehyde 1a. (c) Purification of peptide aldehyde “mini” library containing 16 sequences, stacked chromatograms (DAD) from hydrazide purification steps, and table displaying the retention time of each linear peptide aldehydes (L1-L16) from the purified “mini” library.