Figure 4.

Linearization and derivatization of cyclic peptides. (a) Reaction scheme of one-pot linearization and derivatization of cyclic model peptide 2a to derivatized linear peptide 3a. (b) Stacked chromatograms displaying time-point analysis of one-pot linearization and derivatization of cyclic model peptide 2a to derivatized linear peptide 3a. (c) Scheme for the linearization of library of cyclic peptides by DeClick chemistry and derivatization of resulting linear peptide aldehyde library with mass enhancers. (d) Extracted ion chromatograms of derivatized a mini cyclic peptide library