Figure 5.

Affinity selection experiments with mixture of cyclic peptides. (a) General workflow for HPSEC affinity selection and identification of cyclic peptide binders of 12ca5 antibody from a cyclic peptide library by PEPTIC workflow using linearization of cyclic peptide binders, derivatization of linear peptide aldehydes followed by analysis using nano LC-MS/MS. (b) Representation of microscale thermophoretic assay and analysis of bound from unbound. (c) Individual cyclic peptides based on enriched peptide sequences using HPSEC affinity selection against 12ca5 antibody showed qualitative binding to 12ca5 antibody as analyzed by microscale thermophoretic assay, pink: peptide only; blue: peptide + protein. Microscale thermophoretic assay with random sequence not enriched by PEPTIC workflow did not show any binding with 12ca5 antibody