FIG. 8.

Bcl-2 cannot protect against p21-mediated apoptosis in cervical cancer cells. (A) Expression of Bcl-2 protein. Cellular proteins were extracted, then separated by a SDS-PAGE, and subjected to immunoblot analysis with antibody recognizing Bcl-2 (Santa Cruz). Lanes 1 and 2 represent SiHa cells and SiHa/bcl2 cells (which contain the bcl-2 gene). (B) Low-molecular-weight DNA was harvested from attached and detached SiHa/bcl2 cells which were infected with sense p21 (lane 2) or antisense p21 (lane 1) adenovirus and then analyzed by electrophoresis on a 1.4% agarose gel. (C) SiHa cells with or without Bcl-2 protein infected with sense p21 or antisense p21 adenovirus for 2 days were stained with acridine orange and observed with a fluorescence microscope. Panels a and b represent SiHa cells infected with sense p21 and antisense p21 adenovirus, respectively. Panels c and d represent SiHa/bcl2 cells infected with sense p21 and antisense p21 adenovirus, respectively. The apoptotic cells were observed in panels a and c (arrowheads).