Fig. 6.

Silencing of ACSL1 abolished the lncRNA H19-mediated suppression of TGF-β1-induced EMT and inflammatory response. (A) Western blotting detection and quantification of (B) E-cadherin, (C) α-smooth muscle actin and (D) fibronectin in HK-2 cells with different treatments (n = 3). (E, F) Western blotting detection and quantification of nuclear factor-κB P65 in cells with various treatments (n = 3). Data were presented as mean ± standard deviation, *p < 0.05 and **p < 0.01. α-SMA, α-smooth muscle actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TGF-β1, transforming growth factor-β1; si-NC, negative control siRNA; si-ACSL1, long-chain acyl-CoA synthetase 1 siRNA; P65, nuclear factor-κB P65.