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. 2024 May 9;9(4):1120–1132. doi: 10.1016/j.ncrna.2024.05.002

Fig. 7.

Fig. 7

lncRNA H19 mediates the expression of ACSL1 by microRNA-130a-3p. (A) Alignment between microRNA-130a-3p and lncRNA H19 or 3′-untranslated region of ACSL1. (B) A luciferase reporter assay was conducted in 293T cells co-transfected with negative control mimics or microRNA-130a-3p mimics and wild-type or mutant lncRNA H19 reporter plasmids (n = 3). (C) RNA pull-down assay was performed in HK-2 cells transfected with biotinylated negative control mimics or microRNA-130a-3p mimics. Levels of lncRNA H19 in RNA precipitants was assessed by quantitative real-time polymerase chain reaction (n = 3). (D) A luciferase reporter assay was carried out in 293T cells co-transfected with negative control mimics or microRNA-130a-3p mimics and wild-type or mutant ACSL1 3′-untranslated region reporter plasmids (n = 3). (E, F) HK-2 cells were co-transfected with adenoviruses expressing lncRNA H19 or control adenoviruses and negative control mimics or microRNA-130a-3p mimics before being stimulated with TGF-β1, and ACSL1 levels were detected using Western blotting (n = 3). Data were presented as mean ± standard deviation, *p < 0.05, **p < 0.01 and ***p < 0.001. ACSL1, long-chain acyl-CoA synthetase 1; NC mimics, negative control mimics; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TGF-β1, transforming growth factor-β1.