Table 5.

Production of test specimen.

Work order	Work process	Special features for
		PETG, PETG + nHA, PETG + nFA	PLA, PLA + nHA, PLA + nFA
1.	used filament as carrier material	colorFabb_HT clear, 1.75 mm (colorFabb B.V., Netherlands)	extrudr PLA NX2 white (Extrudr FD3D GmbH, Austria)
2.	mount suitable printing sheet (Prusa i3 MK3s+)	double-sided textured PEI powder-coated spring steel sheet for Original Prusa i3 MK3/S/+ and MK2.5/S heatbeds (Prusa Research a.s., Czech Republic)	spring steel sheet with smooth double-sided PEI for the Original Prusa i3 MK3/S and MK2.5/S heatbeds (Prusa Research a.s., Czech Republic)
3.	place the filament in container using nitrile examination gloves	PETG, PETG + nHA, PETG + nFA	PLA, PLA + nHA, PLA + nFA
4.	mount collection container on the printer frame (Prusa i3 MK3s+)
5.	change of filament (Prusa i3 MK3s+)
6.	print test specimens for 1800 s, then stop the printing process and discard the test specimens
7.	print test specimen with following printing parameters	Original Prusa i3 MK3s + 0.25 nozzle	Original Prusa i3 MK3s + 0.25 nozzle
	nozzle temperature	270 °C	205 °C
	heatbed temperature	100 °C	60 °C
	number of perimeters	3	3
	top solid layers	8	8
	bottom solid layers	8	8
	layer thickness	0.05 mm	0.05 mm
	fill density	80 %	80 %
	infill type	grid	Grid
	supports	none	none
	printing time	7500 s	7500 s
8.	cooling of printing sheet
9.	remove the test specimen using nitrile examination gloves, storage of the test specimens	store in separate 3 l general purpose bags	store in separate 3 l general purpose bags
10.	clean printing sheet	sterile gauze pad, soaked in water and dish soap	sterile gauze pad, soaked in 99.9 % isopropanol
11.	removal of remaining filament from container
12.	clean container and extruder	sterile gauze pad, soaked in 99.9 % isopropanol	sterile gauze pad, soaked in 99.9 % isopropanol