Figure 6.

Canalicular staining and immunohistochemical analysis of Sost in femoral bone of male Sp7fl/fl and Sp7fl/fl;Col1a1-Cre mice at 10 weeks of age. (A–P) Canalicular staining (A–H) and immunostaining using an anti-Sost antibody (I–P) in trabecular bone (A–D, I–L) and cortical bone (E–H, M−P) of Sp7^fl/fl (A, B, E, F, I, J, M, N) and Sp7^{fl/fl;Col1a1−Cre} (C, D, G, H, K, L, O, P) mice. The boxed regions in A, C, E, G, I, K, M, and O are magnified in B, D, F, H, J, L, N, and P, respectively. The average number of canaliculi in one osteocyte was counted (Q), and the percentage of Sost-positive osteocytes was counted (R) in the trabecular and cortical bone.. n = 4 (Sp7^fl/fl) and n = 5 (Sp7^{fl/fl;Col1a1−Cre}). Arrows show Sost-positive osteocytes and arrowheads show Sost-negative osteocytes in J and L. The sections were then counterstained with methyl green. Scale bars: 200 μm (A, C, E, G, I, K, M, and O); 50 μm (B, D, F, H, J, L, N, and P). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)