Figure 7.

Real-time RT-PCR analyses of osteoblast and osteocyte marker genes, Tnfsf11, Tnfrsf11b, and Ostn (A, B) Real-time RT-PCR analysis of the osteoblast marker genes, Tnfsf11, Tnfrsf11b, and Ostn (A) and osteocyte marker genes and Ostn (B). RNA was extracted from the osteoblast fraction (A) and osteocyte fraction (B) of tibiae in female Sp7fl/fl;Col1a1-Cre and the control (Sp7^fl/+ and Sp7^fl/fl) mice at 10 weeks of age. The values of Sp7^fl/+ and Sp7^fl/fl mice were defined as 1 and relative levels are shown. n = 17 (7 Sp7^fl/+ and 10 Sp7^fl/fl) and n = 10 (Sp7^{fl/fl;Col1a1−Cre}). (C) Sp7 and Ostn expression in osteoblast and osteocyte fractions from the tibiae in male wild-type and Col1a1-Sp7 transgenic (tg) mice at 14 weeks of age. The values of wild-type mice were defined as 1 and relative levels are shown. n = 11 (wild-type) and n = 11 (Sp7 tg). (D) MC3T3-E1 cells were transfected with either pME18-EGFP or pME18-Sp7. The values of pME18-EGFP were defined as 1 and relative levels are shown. n = 4 (pME18-EGFP) and n = 4 (pME18-Sp7). Similar results were obtained in three independent experiments, and representative data are shown.