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. 2024 Jul 17;14:16541. doi: 10.1038/s41598-024-67309-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Effect of RferSNMPu1 gene silencing in the electroantennogram responses (EAG) of Rhynchophorus ferrugineus in three experimental groups: Lab control, dsRNA control, and dsRNA RferSNMPu1 against three stimuli ferrugineol, ferrugineone, and ethyl acetate. EAG responses significantly differ from the control groups marked with (*). Error bars represent SEM. (B) Representative EAG responses from lab control, dsRNA control, and dsRNA RferSNMPu1 group of insects with respective stimuli Pheromones: ferrugineol and ferrugineone and kairomone: ethyl acetate. Measurements were performed at 10 mV and 15 s intervals.