Table 2.
Top 5 miRNAs Associated With Each CVD Risk Factor in the FHS
| CVD risk factor | miRNA | No. full | Effect size | SE | P value | FDR‐adjusted P value |
|---|---|---|---|---|---|---|
| Systolic blood pressure | miR‐365a‐3p | 3341 | −0.775 | 0.169 | 4.68E‐06 | 0.000772 |
| miR‐193b‐3p | 2721 | −0.645 | 0.150 | 1.70E‐05 | 0.00140 | |
| miR‐20b‐5p | 4134 | 0.511 | 0.127 | 5.47E‐05 | 0.00301 | |
| miR‐16‐5p | 4376 | 0.378 | 0.0957 | 7.78E‐05 | 0.00321 | |
| miR‐25‐3p | 4389 | 0.414 | 0.113 | 0.000240 | 0.00790 | |
| Diastolic blood pressure | miR‐193b‐3p | 2721 | −0.428 | 0.103 | 3.18E‐05 | 0.00524 |
| miR‐130a‐3p* | 4144 | 0.228 | 0.0738 | 0.00200 | 0.0825 | |
| miR‐122‐5p* | 4382 | −0.214 | 0.0682 | 0.00170 | 0.0825 | |
| miR‐365a‐3p* | 3341 | −0.372 | 0.118 | 0.00158 | 0.0825 | |
| miR‐34a‐5p* | 3612 | −0.305 | 0.103 | 0.00319 | 0.105 | |
| Triglycerides | miR‐122‐5p | 4382 | −0.0292 | 0.00315 | 2.44E‐20 | 4.02E‐18 |
| miR‐193b‐3p | 2721 | −0.0412 | 0.00479 | 1.35E‐17 | 1.11E‐15 | |
| miR‐193a‐5p | 4261 | −0.0288 | 0.00399 | 6.68E‐13 | 3.68E‐11 | |
| miR‐365a‐3p | 3341 | −0.0378 | 0.00546 | 5.22E‐12 | 2.15E‐10 | |
| miR‐194‐5p | 4047 | −0.0279 | 0.00436 | 1.65E‐10 | 5.43E‐09 | |
| Total cholesterol | miR‐126‐3p | 4265 | −0.0112 | 0.00172 | 7.29E‐11 | 1.20E‐08 |
| miR‐191‐5p | 4259 | −0.00880 | 0.00141 | 4.52E‐10 | 2.14E‐08 | |
| miR‐425‐3p | 4064 | −0.00961 | 0.00155 | 5.77E‐10 | 2.14E‐08 | |
| miR‐425‐5p | 4256 | −0.00883 | 0.00143 | 6.48E‐10 | 2.14E‐08 | |
| miR‐625‐3p | 4266 | −0.00888 | 0.00142 | 4.69E‐10 | 2.14E‐08 | |
| High‐density lipoprotein | miR‐204‐5p* | 4131 | −0.315 | 0.112 | 0.00493 | 0.409 |
| miR‐375‐3p* | 4196 | −0.339 | 0.115 | 0.00324 | 0.409 | |
| miR‐301a‐3p* | 3478 | −0.334 | 0.133 | 0.0121 | 0.622 | |
| miR‐502‐3p* | 2635 | −0.436 | 0.186 | 0.0187 | 0.622 | |
| miR‐532‐3p* | 2718 | −0.364 | 0.153 | 0.0173 | 0.622 | |
| Fasting blood glucose | miR‐192‐5p | 4130 | −0.00647 | 0.00136 | 1.93E‐06 | 0.000114 |
| miR‐193a‐5p | 4263 | −0.00594 | 0.00123 | 2.05E‐06 | 0.000114 | |
| miR‐193b‐3p | 2723 | −0.00789 | 0.00161 | 1.00E‐06 | 0.000114 | |
| miR‐122‐5p | 4384 | −0.00441 | 0.000995 | 9.61E‐06 | 0.000399 | |
| miR‐365a‐3p | 3343 | −0.00684 | 0.00181 | 0.000155 | 0.00515 | |
| Body mass index | miR‐122‐5p | 4384 | −0.357 | 0.0411 | 4.97E‐18 | 8.26E‐16 |
| miR‐193b‐3p | 2723 | −0.462 | 0.0611 | 5.19E‐14 | 4.31E‐12 | |
| miR‐193a‐5p | 4263 | −0.364 | 0.0520 | 3.01E‐12 | 1.67E‐10 | |
| miR‐365a‐3p | 3343 | −0.489 | 0.0702 | 4.02E‐12 | 1.67E‐10 | |
| miR‐101‐3p | 4155 | 0.269 | 0.0512 | 1.61E‐07 | 5.33E‐06 | |
| Current cigarette smoking | miR‐150‐5p | 4382 | −0.334 | 0.0711 | 2.71E‐06 | 0.000339 |
| miR‐152‐3p | 3743 | −0.300 | 0.0631 | 2.03E‐06 | 0.000506 | |
| miR‐125a‐5p | 4360 | −0.307 | 0.0707 | 1.42E‐05 | 0.00118 | |
| miR‐34a‐5p | 3613 | −0.277 | 0.0666 | 3.14E‐05 | 0.00130 | |
| miR‐584‐5p | 4237 | −0.315 | 0.763 | 3.72E‐05 | 0.00132 | |
| Former cigarette smoking | miR‐99a‐3p* | 1200 | −0.254 | 0.0845 | 0.00268 | 0.671 |
| miR‐744‐5p* | 3375 | 0.0182 | 0.0580 | 0.754 | 0.981 | |
| miR‐425‐3p* | 4064 | 0.0184 | 0.0581 | 0.752 | 0.984 | |
| miR‐15a‐5p* | 4356 | −0.0215 | 0.0671 | 0.749 | 0.985 | |
| miR‐29a‐3p* | 4265 | 0.0242 | 0.0543 | 0.657 | 0.989 |
Associations after adjustment for age, sex, cohort, and lipid levels. CVD indicates cardiovascular disease; FDR, false discovery rate; FHS, Framingham Heart Study; and miRNA, microRNA.
*
Association not statistically significant (FDR>0.05).