Table 1.
35 significantly changed proteins
| Protein name | Protein ID | Coverage (%) | Spectrum counts | Unique peptides | Amin acids | Molar weights | SAL abundance | LYZ+SAL Abundance |
Fold changes | p-Value | Function |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Siderophore- interacting protein | yqjH | 10.98 | 4 | 3 | 255 | 28.9 | 184.4333 | 0 | 0 | 0.019824 | Ferric reductase in different iron assimilation pathways |
| N-acetyltransferase | 21.71 | 3 | 3 | 175 | 19.1 | 200 | 0 | 0 | 0.033553 | Transfer actyl group from intercellular into bacteria | |
| Cytoplasmic protein | 22.37 | 3 | 1 | 76 | 8.3 | 200 | 0 | 0 | 0.046521 | Cytoplasmic proteins | |
| Serine protease | 11.72 | 6 | 3 | 273 | 29.1 | 173.9667 | 14.86667 | 0.1 | 0.042902 | Hydrolaysis serine | |
| PadR family transcriptional regulator | yqji | 14.88 | 9 | 3 | 215 | 24.2 | 173.5667 | 26.43333 | 0.2 | 0.005656 | Represses the expression of YqjH which is involved in iron homeostasis under excess nickel conditions. |
| Ethanolamine utilization microcompartment protein EutM | EutM | 37.5 | 11 | 3 | 96 | 9.8 | 173.1333 | 26.86667 | 0.2 | 0.046791 | A transcriptional repressor, is important for preventing expression when other preferred sources of energy are available |
| Ribosomal RNA small subunit methyltransferase I | yraL | 24.74 | 15 | 6 | 287 | 31.5 | 173.3 | 26.7 | 0.2 | 0.042308 | Active on the assembled 30S subunit |
| Conjugal transfer protein, ATP-binding | traJ | 14.4 | 14 | 5 | 382 | 42.5 | 173.1 | 26.9 | 0.2 | 0.038769 | Activates the PY promoter and the transcription of the operon |
| Putative epimerase LsrE | LsrE | 19.29 | 11 | 4 | 254 | 27.6 | 159 | 41 | 0.3 | 0.01799 | Putative epimerase, is the final protein in the A-2 quorum sensing pathway yet to be characterize |
| Copper homeostasis protein CutF (Lipoprotein nlpE) | nlpE | 9.01 | 9 | 3 | 233 | 25.2 | 151.2333 | 48.73333 | 0.4 | 0.002936 | Involved in both copper efflux and the delivery of copper to copper-dependent enzymes |
| D-threonate kinase | 18.68 | 10 | 6 | 423 | 45 | 153.2667 | 46.7 | 0.5 | 0.044597 | A kinase involved in the catabolic pathway of D-threonate | |
| Bacteriophage repressor protein cI | cl | 19.37 | 11 | 3 | 191 | 20.5 | 143.2333 | 56.73333 | 0.5 | 0.018677 | Prevents lytic growth by directly repressing two promoters needed to express lytic functions, PL and PR |
| Type III secretion system effector protein, deubiquitinase | sseL | 16.4 | 18 | 5 | 317 | 35.5 | 140.9 | 59.1 | 0.5 | 0.008821 | Translocated into host cells during intracellular infection |
| Cytosine deaminase | codA | 34.98 | 46 | 11 | 426 | 47.6 | 137.5667 | 62.46667 | 0.6 | 0.025808 | Allows the cell to utilize cytosine for pyrimidine nucleotide synthesis |
| Protease 3 | 15.7 | 56 | 13 | 962 | 107.4 | 133.3 | 66.7 | 0.7 | 0.031043 | Endopeptidase (alkaline protease) of the serine protease family and cleaves proteins in the tissue section | |
| FadR family transcriptional regulator | yieP | 23.58 | 7 | 5 | 229 | 26 | 81.43333 | 118.5667 | 1.8 | 0.027262 | Regulates the expression of genes encoding fatty acid biosynthetic and degrading enzymes |
| Histidine transport ATP-binding protein | hisP | 14.34 | 9 | 3 | 258 | 28.8 | 80.9 | 119.1 | 1.8 | 0.048651 | Part of the ABC transporter complex HisPMQJ involved in histidine transport |
| tRNA/tmRNA (uracil- C(5))-methyltransferase | trmA | 20.49 | 19 | 6 | 366 | 41.9 | 78.23333 | 121.7333 | 2.1 | 0.043658 | Dual-specificity methyltransferase that catalyzes the formation of 5- methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 in tmRNA |
| Asparagine synthase B | asnB | 25.27 | 52 | 13 | 554 | 62.5 | 71.03333 | 128.9667 | 2.2 | 0.021078 | Catalyzes the ATP- dependent conversion of aspartate into asparagine, using glutamine as a source of nitrogen |
| Hypothetical membrane protein | yijP | 11.36 | 10 | 2 | 132 | 14.8 | 69.63333 | 130.3333 | 2.3 | 0.027148 | Membrane protein |
| Cell invasion protein | invE | 18.82 | 17 | 6 | 372 | 42.4 | 65.36667 | 134.6 | 2.5 | 0.032865 | Triggering of intracellular events that lead to microbial internalization |
| Type I restriction enzyme | hsdS | 8.1 | 10 | 3 | 469 | 51.9 | 63.86667 | 136.1667 | 2.6 | 0.000564 | Recognizes the target DNA sequence |
| Major outer membrane lipoprotein Lpp | Lpp1 | 62.82 | 85 | 6 | 78 | 8.4 | 63.6 | 136.3667 | 2.7 | 0.000564 | Major outer membrane |
| Chaperone protein SicP | slcP | 6.9 | 3 | 1 | 116 | 12.9 | 58.86667 | 141.1667 | 3 | 0.03567 | Binds SptP, which in its absence completely is degraded within tie bacterial cytoplasm |
| Peptidoglycan-binding protein LysM | lysM | 52.35 | 17 | 6 | 149 | 16.1 | 55.73333 | 144.2667 | 3.1 | 0.048491 | Outer membrane protein, activates a common signal transduction pathway response |
| N-acetyl-gamma-glutamyl-phosphate reductase | argC | 7.19 | 4 | 2 | 334 | 35.9 | 55.4 | 144.6 | 3.1 | 0.031262 | Catalyze NADPH- dependent reductive dephosphorylation of N-acetyl-gamma- glutamyl-phosphate to N-acetylglutamate- gamma-semialdehyde |
| UPF0194 membrane protein YbhG | YbhG | 45.32 | 34 | 12 | 331 | 36.3 | 55.96667 | 144.0667 | 3.2 | 0.023133 | Membrane protein |
| Abequosyltransferase RfbV | RfbV | 16.22 | 17 | 6 | 333 | 38.6 | 56.36667 | 143.6333 | 3.3 | 0.033607 | Catalyzes the transfer of CDP-abequose on D-mannosyl-L- rhamnosyl-D- galactose-1- diphospholipid to yield D-abequosyl-D-mannosyl-rhamnosyl-D-galactose-1-diphospholipid |
| Cysteine/glutathione ABC transporter ATP- binding protein/permease CydC | CydC | 15.36 | 12 | 6 | 573 | 63 | 22.33333 | 177.6667 | 9 | 0.032605 | It is involved in the export of glutathione from the cytoplasm to the periplasm and is required for the assembly of both cytochrome c and cytochrome bd |
| ATP-independent periplasmic protein-refolding chaperone | spy | 16.15 | 5 | 3 | 161 | 18.2 | 20.66667 | 179.3333 | 10 | 0.03805 | Folding landscape of the substrate in determining chaperone mechanisms |
| Aspartate--ammonia | asnA | 17.88 | 11 | 5 | 330 | 36.8 | 22.76667 | 177.2667 | 10.6 | 0.033566 | Catalyzes the ATP- dependent conversion of Asn |
| Hypothetical membrane protein (SPI- 6 associated) | sclP | 1.61 | 2 | 1 | 434 | 47.3 | 0 | 200 | 100 | 0.029191 | Membrane protein |
| H(+)/Cl(−) exchange transporter ClcA | clcA | 2.75 | 1 | 1 | 473 | 50.4 | 0 | 200 | 100 | 0.009301 | Proton-coupled chloriode transporter |
| Oligopeptide ABC transporter permease OppC | oppC | 7.95 | 2 | 2 | 302 | 33.1 | 0 | 200 | 100 | 0.020463 | Binds peptides of 3 amino acids to at least 16 amino acids in length independent of amino acid content |
| TDC operon transcriptional activator | tdcA | 6.73 | 7 | 2 | 312 | 34.8 | 0 | 200 | 100 | 0.023254 | Membrane protein |