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Annexin V Red binds exposed phosphatidyl serine on the outer leaflet of apoptotic cell membranes. Cytotox Green integrates into cells with compromised membrane integrity and binds DNA.
| Treatment | # of wells | Media Volume | Annexin | 100 μM Cytotox | ||
|---|---|---|---|---|---|---|
| Dilution | Volume | Dilution | Volume | |||
| Multiplex | 60 | 6.6 mL | 1:400 | 16.5 μL | 200 nM | 13.2 μL |
